The cellulosome complex

  1. Alon Karpol, Yoav Barak, Raphael Lamed, Yuval Shoham and Edward A. Bayer. Functional asymmetry in cohesin binding belies inherent symmetry of the dockerin module: Insight into cellulosome assembly revealed by systematic mutagenesis. Biochem. J. 410:331-338, 2008
  2. Bayer, E. A., H. Chanzy, R. Lamed, and Y. Shoham. Cellulose, cellulases and cellulosomes. Curr. Opin. Struct. Biol. 8:548-557, 1998.
  3. Bayer, E. A., L.J.W. Shimon, Y. Shoham, and R. Lamed. Cellulosomes-structure and ultrastructure. J. Structural Biology 124:221-234, 1998.
  4. Berdichevsky, Y., R. Lamed, D. Frenkel, U. Gophna, E. A. Bayer, S. Yaron, Y. Shoham, and I. Benhar. Matrix-assisted refolding of single-chain Fv -cellulose binding domain fusion proteins. Protein Express. Purif. 17:249-259, 1999.
  5. Boisset, C., Chanzy, H., Henrissat, B., Lamed, R., Shoham, Y. and Bayer, E. A. Digestion of crystalline cellulose substrates by the Clostridium thermocellum cellulosome: Structural and morphological aspects. Biochem. J. 340:829-835, 1999.
  6. Borovok, Ilya, Hamutal Kahel-Raifer, Sadanari Jindou Liat Bahari, Yakir Nataf, Yuval Shoham, Edward Bayer, Raphael Lamed. Unique set of putative membrane-associated anti-sigma factors in Clostridium thermocellum suggests a novel extracellular carbohydrate-sensing mechanism involved in gene regulation. FEMS Microbiol. Lett. 308:84–93, 2010.
  7. David Nakar, Tal Handelsman, Yuval Shoham, Henri-Pierre Fierobe, Jean-Pierre Belaich, Ely Morag Raphael Lamed and Edward A. Bayer. Pinpoint mapping of recognition residues on the cohesin surface by progressive homologue swapping. J. Biol. Chem. 279:42881-42888, 2004.
  8. Ding, S-Y., E.A. Bayer, D. Steiner, Y. Shoham, and R. Lamed. A novel cellulosomal scaffoldin from Acetivibrio cellulolyticus which contain a Family 9 glycosyl hydrolase. J. Bacteriol. 181:6720-6729, 1999.
  9. Ding, S-Y., E.A. Bayer, D. Steiner, Y. Shoham, and R. Lamed. A scaffoldin of the Bacteroides cellulosolvens cellulosome that contains eleven type-II cohesins. J. Bacteriol. 182:4915-4925, 2000.
  10. Edward A. Bayer, Jean-Pierre Belaich, Yuval Shoham, and Raphael Lamed. The cellulosomes: multi-enzyme machines for degradation of plant cell wall polysaccharides. Ann. Rev. Microbiol. 58:521-554. 2004
  11. Haimovitz, Rachel, Yoav Barak, Ely Morag, Yuval Shoham, Milana Voronov-Goldman, Raphael Lamed, and Edward A. Bayer. Cohesin-dockerin microarray: diverse specificities between two complementary families of interacting protein modules. Proteomics. 8:968-979, 2008
  12. Henri-Pierre Fierobe, Adva Mechaly, Chantal Tardif, Anne Belaich, Raphael Lamed, Yuval Shoham, Jean-Pierre Belaich, and Edward A. Bayer. Design and production of active cellulosome chimeras: selective incorporation of dockerin-containing enzymes into defined functional complexes. J. Biol. Chem. 276:21257-21261, 2001.
  13. Henri-Pierre Fierobe, Edward A. Bayer, Chantal Tardif, Mirjam Czjzek, Adva Mechaly, Anne Belaich, Raphael Lamed, Yuval Shoham, and Jean-Pierre Belaich. Degradation of cellulose substrates by cellulosome chimeras: substrate targeting versus proximity of enzyme components. J. Biol. Chem. 277:49621-49630, 2002.
  14. Jonathan Caspi, Diana Irwin, Raphael Lamed, Yuval Shoham, Henri-Pierre Fierobe, David B. Wilson, and Edward A. Bayer. Thermobifida fusca family-6 cellulases as potential designer cellulosome components. Biocatalysis and Biotransformation 24:3-12, 2006.
  15. Kauffmann, C., O. Shoseyov, E. A. Bayer, R. Lamed, Y. Shoham, and R. T. Mandelbaum. A novel methodology for enzymatic removal of atrazine from water by CBD-fusion protein immobilized on cellulose. Environ. Sci. Technol. 34:1292-1296, 2000
  16. Lamed, R., Kenig, R., Morag, E., Yaron, S., Shoham, Y. and Bayer, E. A. Nonproteolytic cleavage of aspartyl proline bonds in the cellulosomal scaffoldin subunit from Clostridium thermocellum. Appl. Biochem. Biotechnol. 90:67-74, 2001.
  17. Liat Bahari, Yuval GIlad, Ilya Borovok, Hamutal Kahel-Raifer, Bareket Dassa, Yakir Nataf, Yuval Shoham, Raphael Lamed, Edward A. Bayer. Glycoside hydrolases as components of putative carbohydrate biosensor proteins in Clostridium thermocellum J. Ind. Microbiol. Biotechnol. 38:825–832, 2011.
  18. Linda J.W. Shimon, Sandrine Pagès, Anne Belaich, Jean-Pierre Belaich, Edward A. Bayer, Raphael Lamed, Yuval Shoham, and Felix Frolow. Crystal structure of a Family-IIIa scaffoldin CBD from the cellulosome of Clostridium cellulolyticum at 2.2 Å resolution. Acta Cryst D56:1560-1568, 2000.
  19. Marco T. Rincon, Shi You Ding, Martin, Aurilia, Raphael Lamed, Yuval Shoham, Edward A. Bayer, and Harry J. Flint. Novel organization and divergent dockerin specificities in the cellulosome system of Ruminococcus flavefaciens. J. Bacteriol. 185:703-713, 2003.
  20. Mechali, Adva, Henri-Pierre Fierobe, Anne Belaich, Jean-Pierre Belaich, Raphael Lamed, Yuval Shoham, and Edward, A. Bayer. Coheisn-dockerin interaction in cellulosome assembly: a single hydroxyl group of a dockerin domain distinguish between non-recognition and high -affinity recognition. J. Biol. Chem. 276: 9883-9888, 2001.
  21. Mechaly, A., S. Yaron, H-P. Fierobe, A. Belaich, J-P. Belaich, R. Lamed, Y. Shoham, and E. A. Bayer. Cohesin-dockerin recognition in cellulosome assembly: experiment versus hypothesis. Proteins 39:170-177, 2000.
  22. Morag E., A. Lapidot, D. Govorko, R. Lamed, M. Wilchek, E. A. Bayer and Y. Shoham. Expression, purification and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Appl. Enviorn. Microbiol. 61:1980-1986, 1995
  23. Morag, E., S. Yaron, R. Lamed, Y. Shoham, and E. A. Bayer. Dissociation of the cellulosome of Clostridium thermocellum under nondenaturing conditions. J. Biotechnol. 51:235-242, 1996.
  24. Oren Yaniv, Galit Fichman, Ilya Borovok, Yuval Shoham, Edward Bayer, Raphael Lamed, Linda Shimon and Felix Frolow. Fine-structural variance of family 3 carbohydrate-binding modules as extracellular biomass-sensing components of Clostridium thermocellum anti-sigmaI factors. Acta Cryst D (accepted).
  25. Pages, S., A. Belaich, J.P. Beleich, E. Morag, R. Lamed, Y. Shoham, and E. A. Bayer. Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: predication of specificity determinants of the dockerin domain. Proteins 29:517-527, 1997.
  26. Qi Xu, Edward A. Bayer, Milana Goldman, Rina Kenig, Yuval Shoham and Raphael Lamed. Architecture of the Bacteroides cellulosolvens cellulosome: description of a cell-surface anchoring scaffoldin and a Family 48 cellulase. J. Bacteriol. 186:968-977. 2004.
  27. Qi Xu, Shi-You Ding, Wenchen Gao, Rina Kenig, Yuval Shoham, Edward A. Bayer, and Raphael Lamed. The cellulosome system of Acetivibrio cellulolyticus includes a novel type of adaptor protein and a cell-surface anchoring protein. J. Bacteriol. 185:4548-4557, 2003.
  28. Qi Xu, Yoav Barak, Rina Kenig, Yuval Shoham, Edward A. Bayer, and Raphael Lamed. A novel Acetivibrio cellulolyticus anchoring scaffoldin that bears divergent cohesins. J. Bacteriol. 186:5782-5789, 2004.
  29. Rachel Gilad, Larisa Rabinovich, Sima Yaron, Edward A. Bayer, Raphael Lamed, Harry J. Gilbert and Yuval Shoham. CelI, a non-cellulosomal family-9 enzyme from Clostridium thermocellum, is a processive endoglucanase that degrades crystalline cellulose. J. Bacteriol. 185:391-398, 2003.
  30. Sadanari Jindou, Qi Xu, Rina Kenig, Michal Shulman, Yuval Shoham, Edward Bayer, and Raphael Lamed. Novel architecture of family-9 glycoside hydrolases identified in cellulosomal enzymes of Acetivibrio cellulolyticus and Clostridium thermocellum. FEMS Lett. 254:308-316, 2006.
  31. Sarah Moraïs, Ely Morag, Yoav Barak, Dan Goldman, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, and Edward A. Bayer. Deconstruction of lignocellulose into soluble sugars by native and designer cellulosomes. MBio Volume 3 Issue 6 e00508-12, 2012.
  32. Sarah Moraïs, Orly Salama-Alber, Yoav Baraka, Yitzhak Hadar, David B. Wilson, Raphael Lamed, Yuval Shoham and Edward A. Bayer. Functional association of the catalytic and ancillary modules dictates enzymatic activity in a family-43 -xylosidase. J. Biol. Chem. 287:9213-9221, 2012.
  33. Sarah Moraïs, Yoav Barak, Jonathan Caspi, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, and Edward A. Bayer. Degradation of a complex cellulosic substrate by designer cellulosomes: Contribution of a xylan-binding module. Appl. Enviorn. Microbiol. 76:3787-3796, 2010.
  34. Sarah Moraïs, Yoav Barak, Yitzhak Hadar, David Wilson, Yuval Shoham, Raphael Lamed, and Edward Bayer. Assembly of xylanases into designer cellulosomes promotes efficient hydrolysis of the xylan component of a natural recalcitrant cellulosic substrate. MBio 2(6):e00233-11, 2011.
  35. Sarah Moraïsa, Yoav Barak, Jonathan Caspia, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, and Edward A. Bayer. Cellulase-xylanase synergy in designer cellulosome for enhanced degradation of a complex cellulosic substrate. mBio 1(5):e00285-10. doi:10.1128/mBio.00285-10, 2010.
  36. Shi-You Ding, Marco T. Rincon, Raphael Lamed, Jennifer C. Martin, Sheila I. McCrae, Vincenzo Aurilia, Yuval Shoham, Edward A. Bayer, and Harry J. Flint. Cellulosomal scaffoldin-like proteins from Ruminococcus flavefaciens. J. Bacteriol. 183:1945-1953, 2001
  37. Shimon, L. J.W., E. A. Bayer, E. Morag, R. Lamed, S. Yaron, Y. Shoham, and F. Frolow. Three-dimensional crystal structure of a cohesin domain of the cellulosome from Clostridium thermocellum. Structure 5:381-390, 1997.
  38. Shimon, L. J.W., F. Frolow, S. Yaron, R. Lamed, E. Morag, E. A. Bayer, and Y. Shoham. Crystallization and preliminary X-ray analysis of a cohesin domain of the cellulosome from Clostridium thermocellum. Acta Crystallogr. D. 53:114-115, 1997.
  39. Shoham, Y., R. Lamed, and E.A. Bayer. The cellulosome concept as an efficient microbial strategy for the degradation of insoluble polysaccharides. Trends in Microbiol. 7:275-281, 1999.
  40. Steven Brown, Raphael Lamed, Ely Morag, Ilya Borovok, Yuval Shoham, Dawn Klingeman, Courtney Johnson, Zamin Yang, Miriam Land, Sagar Utturkar, and Edward Bayer. Draft genome sequences for Clostridium thermocellum wild-type Strain YS and derived cellulose adhesion-defective mutant strain AD2. J. Bacteriol 194(12):3290-1, 2012.
  41. Tal Burstein, Michal Shulman, Sadanari Jindou, Svetlana Petkun, Felix Frolow, Yuval Shoham, Edward A. Bayer, and Raphael Lamed. Physical association of the catalytic and helper modules of a family-9 glycoside hydrolase is essential for activity. FEBS Lett. 583:879-884, 2009.
  42. Tal Handelsman, Yoav Barak, David Nakar, Adva Mechaly, Raphael Lamed, Yuval Shoham, and Edward A. Bayer. Cohesin-dockerin interaction in cellulosome assembly: A single Asp-to-Asn mutation disrupts high-affinity cohesin-dockerin binding. FEBS Lett. 572:195-200, 2004.
  43. Tali W. Dror, Adi Rolider, Edward A. Bayer, Raphael Lamed and Yuval Shoham. Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs from that of a prominent cellulosomal xylanase. J. Bacteriol. 187:2261-2266, 2005.
  44. Tali W. Dror, Adi Rolider, Edward A. Bayer, Raphael Lamed and Yuval Shoham. Regulation of expression of scaffoldin-related genes in Clostridium thermocellum. J. Bacteriol. 185:5109-5116, 2003.
  45. Tali W. Dror, Ely Morag, Adi Rolider, Edward A. Bayer, Raphael Lamed and Yuval Shoham. Regulation of the cellulosomal celS (cel48A) gene of Clostridium thermocellum is growth-rate dependent. J. Bacteriol. 185:2042-3048, 2003.
  46. Tormo, J., R. Lamed, A. J. Chirino, E. Morag, E. A. Bayer, Y. Shoham, and T. A. Steitz. Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. EMBO J. 15: 5739-5751, 1996.
  47. Yakir Nataf, Liat Bahari, Hamutal Kahel-Raifer, Ilya Borovok, Raphael Lamed, Edward A. Bayer, Abraham L. Sonenshein and Yuval Shoham. Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors. Proc. Natl. Acad. Sci. USA 107:18646–18651, 2010.
  48. Yakir Nataf, Sima Yaron, Frank Stahl, Raphael Lamed, Edward A. Bayer, Thomas-Helmut Scheper, Abraham L. Sonenshein, and Yuval Shoham. Cellodextrin and laminaribiose ABC transporters in Clostridium thermocellum. J. Bacteriol. 191:203-209,2009.
  49. Yaron, S., E. Morag, E.A. Bayer, R. Lamed and Y. Shoham. Expression, purification and subunit-binding properties of cohesins 2 and 3 of the Clostridium thermocellum cellulosome. FEBS 360:121-124, 1995.
  50. Yaron, S., L. J. W. Shimon, F. Frolow, R. Lamed, E. Morag, Y. Shoham, and E. A. Bayer. Expression, purification, and crystallization of a cohesin domain from the cellulosome of Clostridium thermocellum. J. Biotechnol. 51:243-249, 1996.
  51. Yoav Barak, Tal Handelsman, David Nakar, Adva Mechaly, Raphael Lamed, Yuval Shoham, and Edward A. Bayer. Matching fusion-protein systems for affinity analysis of two interacting families of proteins: The cohesin-dockerin interaction. J. Mol. Recognit. 18:491-501, 2005.